The usage method and operation steps of laboratory small-scale bioreactors
A laboratory small-scale bioreactor is a laboratory desktop bioreactor that is flexible, easy to operate, and occupies a very small area. It adopts a fully computerized touch screen control system, displays on a color touch screen, and has powerful expansion functions. It supports up to 24 fermentation parameter controls and is an experimental platform for studying fermentation process conditions and precision cultivation.
Main components of laboratory small bioreactors
The tank is mainly used for cultivating and fermenting various bacterial cells, with good sealing;
There is a stirring slurry in the tank, which is used for continuous stirring during the fermentation process; Sparger with bottom ventilation, used to introduce air or oxygen required for bacterial growth;
There are control sensors on the top plate of the tank, commonly including pH electrodes and DO electrodes, used to monitor changes in the pH and DO of the fermentation broth during the fermentation process; Controller, used to display and control fermentation conditions, etc.
The usage method and operation steps of laboratory small-scale bioreactors
1. Calibrate pH electrode and dissolved oxygen electrode;
2. Sterilization of tanks. Add the culture medium into the tank as needed, seal it as required, and place the tank in a large sterilization pot for sterilization;
After the tank has cooled down, place it on the fermentation platform and install it properly;
3. Turn on the cooling water, turn on the power of the air pump, connect the ventilation pipe to start ventilation, and adjust the intake knob to adjust the ventilation volume appropriately; Turn on the power of the bioreactor, set the temperature pH、 Set the stirring speed and the dissolved oxygen electrode;
4. After the temperature stabilizes and all parameters are correct, connect the pre shaken seeds, start fermentation timing, and start recording various parameters;
5. After fermentation, clean the tank and electrodes, and insert the electrodes into a triangular flask for later use;
6. Fill in the registration book as required and have it signed by the person in charge.
